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In addition, the cellular location of the interacting proteins is determined.

We used this strategy to map a signal transduction network that controls initiation of translation in eukaryotes.

We report an experimental strategy that allows for detection of protein interactions and functional assays with a single reporter system.

Interactions among biochemical network component proteins are detected and probed with stimulators and inhibitors of the network.

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The second step is to perform functional assays in model cells or whole organisms from which the genes in question were derived.Rapid progress in genome projects is leading to the identification or prediction of a huge number of genes, but only a fraction of gene functions can be inferred from primary gene sequences.A first step in defining the function of a gene is to determine its interactions with other gene products. Wiley Online Library requires cookies for authentication and use of other site features; therefore, cookies must be enabled to browse the site.Detailed information on how Wiley uses cookies can be found in our Privacy Policy.

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