Elucidating pathway arsenic methylation

Rated 4.18/5 based on 959 customer reviews

Multiple-Sequence Alignments of Human AS3MT and Eight Animal Homologues Illustrate Conserved Motifs I, II, and III, Conserved Asp at I′, and Five Conserved Cys Residues.Underlined sequence is Ubi E methyltransferase domain.In particular, these studies show that many animal species convert i As into MAs and DMAs, which are excreted in urine.Determining the basis for this conversion of i As into methylated species depended on development of strategies to isolate, purify, and characterize the molecules that are involved in each step of the pathway that leads from i As to its methylated metabolites and development of sufficiently sensitive and specific analytical methods to identify intermediates and products formed in the methylation pathway.

elucidating pathway arsenic methylation-75

Interactions between Ado Met and these conserved amino acid motifs are critical for methyl group transfer to substrate (10).The AS3MT protein was the first As methyltransferase to be sufficiently purified to permit cloning of the corresponding gene (7, 8).Sequence information on rat AS3MT has provided a starting point to seek a broader perspective on its structure and function as an As methyltransferase, using genomic data to investigate the occurrence of homologous genes across a wide range of species.Intermediates and products formed in this pathway may be more reactive and toxic than inorganic arsenic.Like all metabolic pathways, understanding the pathway for arsenic methylation involves identification of each individual step in the process and the characterization of the molecules which participate in each step.

Leave a Reply